UBE2L3 抗体 (C-Term)
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Quick Overview for UBE2L3 抗体 (C-Term) (ABIN374293)
抗原
See all UBE2L3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-EFTKKYGEKR PVD
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特异性
- This antibody detects UBE2L3 / UBCH7 at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Cow.
Species reactivity (tested):Human, Mouse and Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_003338.1 Genename: UBE2L3
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应用备注
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Peptide ELISA: 1: 128000. Western blot: 0.5-2 μg/mL. Approx 18 kDa band observed in Hela and Jurkat lysates(predicted MW of 18 kDa according to NP_003338).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
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别名
- UBE2L3 / UBCH7
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背景
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.Synonyms: E2-F1, L-UBC, UBCE7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2 L3, Ubiquitin-protein ligase L3
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基因ID
- 7332
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NCBI登录号
- NP_003338
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UniProt
- P68036
抗原
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