UBE2K 抗体 (C-Term)
Quick Overview for UBE2K 抗体 (C-Term) (ABIN374290)
抗原
See all UBE2K 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SWDVETATEL LLSN
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特异性
- This antibody recognizes UBE2K/HIP2 (C-term). It is expected to recognise all reported isoforms.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Canine (Dog), Pig and Cow.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1. Genename: UBE2K
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anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 109-139) antibody
UBE2K 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4144 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 2-200) antibodyUBE2K 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibodyUBE2K 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 山羊, 马, 兔, 豚鼠, Pig, 猴, Hamster, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibodyVerified UBE2K 适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 1-40) antibodyUBE2K 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1D10 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (C-Term) antibodyUBE2K 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 1-100) antibodyUBE2K 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 51-150) antibodyUBE2K 适用: 大鼠 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (AA 108-157) antibodyUBE2K 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 山羊, 马, 兔, 豚鼠, 小鸡, 非洲爪蟾, Pig, 猴, Hamster, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2K (UBE2K) (N-Term) antibodyUBE2K 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 山羊, 马, 兔, 豚鼠, 小鸡, 非洲爪蟾, Pig, 猴, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit Dilution: 1/2000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in Jurkat cell lysates (CalculatedMW of 22.4 kDa according to NP_005330).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted at (in aliquots) at -20 °C.
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- UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
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别名
- HIP2 / UBE2K
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背景
- The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.Synonyms: E2(25K), E2-25K, HIP-2, Huntingtin-interacting protein 2, LIG, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2 K, Ubiquitin-protein ligase
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基因ID
- 3093
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NCBI登录号
- NP_005330
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UniProt
- P61086
抗原
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