PDIA3 抗体 (C-Term)
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Quick Overview for PDIA3 抗体 (C-Term) (ABIN374286)
抗原
See all PDIA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-EEKPKKKKKA QEDL
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特异性
- This antibody detects PDIA3 at C-term.
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交叉反应 （详细）
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Species reactivity (expected):Rat and Cow.
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptidefrom C Terminus of the protein sequence according to NP_005304.3 Genename: PDIA3
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anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (C-Term) antibody
PDIA3 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 猴, Cow, 兔, Pig, Hamster, 马, 山羊, 绵羊, Avian, Cat, 小鸡, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 1-505) antibodyPDIA3 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibodyPDIA3 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3E1 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 140-505) antibodyPDIA3 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3H5 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (C-Term) antibodyPDIA3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibodyPDIA3 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Map-ERp57 unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) (AA 1-505) antibodyPDIA3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (HRP)PDIA3 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Map-ERp57 HRP
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (FITC)PDIA3 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Map-ERp57 FITC
anti-Protein Disulfide Isomerase Family A, Member 3 (PDIA3) antibody (Biotin)PDIA3 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Map-ERp57 Biotin
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应用备注
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Peptide ELISA: Limit Dilution 1/8000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in human hepatoblastoma HepG2lysates (calculated MW of 56.5 kDa according to NP_005304.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
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别名
- PDIA3
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背景
- ERp57, also known as GRP58, PDIA3, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.Synonyms: Disulfide isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, Protein disulfide-isomerase A3
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基因ID
- 2923
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NCBI登录号
- NP_005304
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UniProt
- P30101
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途径
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
抗原
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