NDC80 抗体 (C-Term)
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Quick Overview for NDC80 抗体 (C-Term) (ABIN374264)
抗原
See all NDC80 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-YEKKATLIKS SEE
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特异性
- This antibody detects NDC80 at C-term.
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交叉反应 (详细)
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_006092.1 Genename: NDC80
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 75-80 kDa band observed in Hela lysate (predicted MWof 70 kDa according to NP_006092).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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别名
- NDC80
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背景
- HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.Synonyms: HEC, HEC1, KNTC2
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基因ID
- 10403
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NCBI登录号
- NP_006092
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UniProt
- O14777
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途径
- Maintenance of Protein Location
抗原
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