IRF8 抗体 (C-Term)
Quick Overview for IRF8 抗体 (C-Term) (ABIN374256)
抗原
See all IRF8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-QRSFFRENQQ ITV
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特异性
- This antibody detects IRF8 / ICSBP1 at C-term.
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交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_002154.1 Genename: IRF8
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anti-Interferon Regulatory Factor 8 (IRF8) (AA 128-426) antibody
IRF8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (N-Term) antibodyIRF8 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 1-327) antibodyIRF8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 122-218) antibodyIRF8 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 35-84) antibodyIRF8 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼, Pig, Bat, 小鸡, Hamster, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (C-Term) antibodyVerified IRF8 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 241-269) antibodyIRF8 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 384-409) antibodyIRF8 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 128-426) antibodyIRF8 适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
anti-Interferon Regulatory Factor 8 (IRF8) (AA 137-426) antibodyIRF8 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 45-50 kDa band seen in K562 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- IRF8 (Interferon Regulatory Factor 8 (IRF8))
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别名
- IRF8 / ICSBP1
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背景
- Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl 2 expression and thus may play a role in chronic myelogenous leukemia.Synonyms: IRF-8, Interferon consensus sequence-binding protein, Interferon regulatory factor 8
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基因ID
- 3394
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NCBI登录号
- NP_002154
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UniProt
- Q02556
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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