Melanophilin 抗体 (C-Term)
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Quick Overview for Melanophilin 抗体 (C-Term) (ABIN374219)
抗原
See all Melanophilin (MLPH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-ARHIFAKPVM AQQP
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特异性
- This antibody detects Melanophilin at C-term.
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交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C-Terminus of the protein sequence according to NP_443748.2. Genename: Mlph
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anti-Melanophilin (MLPH) (C-Term) antibody
Verified MLPH 适用: 小鼠 WB, ELISA, IF, ICC, Pull-Down 宿主: 山羊 Polyclonal unconjugated
anti-Melanophilin (MLPH) (C-Term) antibodyMLPH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 310-539) antibodyMLPH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 301-600) antibodyMLPH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 1-600) antibodyMLPH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 511-560) antibodyMLPH 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 541-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32075 unconjugated
anti-Melanophilin (MLPH) (AA 540-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80 kDa band observed in Mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high proteinloads. Immunofluorescence: Successfully used at 0.5 μg/mL to stain mouse melanocytes, seepicture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). GST-pull down assay: see publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Melanophilin (MLPH)
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别名
- Melanophilin
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背景
- Melanophilin is a member of the exophilin subfamily of Rab effector proteins. It forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: Exophilin-3, Leaden protein, Ln, Mlph, Slac2a, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a
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基因ID
- 171531
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NCBI登录号
- NP_443748
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UniProt
- Q91V27
抗原
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