Melanophilin 抗体 (C-Term)
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- 抗原 See all Melanophilin (MLPH) 抗体
- Melanophilin (MLPH)
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抗原表位
- C-Term
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适用
- 小鼠, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Melanophilin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 序列
- C-ARHIFAKPVM AQQP
- 特异性
- This antibody detects Melanophilin at C-term.
- 交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide from C-Terminus of the protein sequence according to NP_443748.2. Genename: Mlph
- Top Product
- Discover our top product MLPH Primary Antibody
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anti-Melanophilin (MLPH) (AA 310-539) antibody
MLPH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) antibodyMLPH 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (C-Term) antibodyMLPH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (C-Term) antibodyVerified MLPH 适用: 小鼠 WB, ELISA, IF, ICC, Pull-Down 宿主: 山羊 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 301-600) antibodyMLPH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 1-600) antibodyMLPH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 511-560) antibodyMLPH 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Melanophilin (MLPH) (AA 541-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32075 unconjugated
anti-Melanophilin (MLPH) (AA 540-569), (C-Term) antibodyMLPH 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80 kDa band observed in Mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high proteinloads. Immunofluorescence: Successfully used at 0.5 μg/mL to stain mouse melanocytes, seepicture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). GST-pull down assay: see publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Melanophilin (MLPH)
- 别名
- Melanophilin (MLPH 产品)
- 别名
- MGC155106 antibody, LOC100222096 antibody, 2210418F23Rik antibody, 5031433I09Rik antibody, AW228792 antibody, D1Wsu84e antibody, Slac-2a antibody, l(1)-3Rk antibody, l1Rk3 antibody, ln antibody, SLAC2-A antibody, melanophilin antibody, MLPH antibody, mlph antibody, Mlph antibody
- 背景
- Melanophilin is a member of the exophilin subfamily of Rab effector proteins. It forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: Exophilin-3, Leaden protein, Ln, Mlph, Slac2a, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a
- 基因ID
- 171531
- NCBI登录号
- NP_443748
- UniProt
- Q91V27
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