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Iba1 抗体 (C-Term, Isoform 1)

This anti-Iba1 antibody is a 山羊 多克隆 antibody detecting Iba1 in WB, IHC (p) 和 EIA. Suitable for 人, 大鼠, 小鼠 和 Pig.
产品编号 ABIN374206
发货至: 中国

Quick Overview for Iba1 抗体 (C-Term, Isoform 1) (ABIN374206)

抗原

See all Iba1 (IBA1) 抗体
Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

适用

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人, 大鼠, 小鼠, Pig

宿主

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  • 1
山羊

克隆类型

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多克隆

标记

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This Iba1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    C-Term, Isoform 1

    特异性

    This antibody is expected to recognizes Isoform 1 (NP_116573.1) and Isoform 3 (NP_001614.3) of AIF1.

    交叉反应 (详细)

    Species reactivity (expected):Human, Porcine.
    Species reactivity (tested):Mouse, Rat.

    纯化方法

    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3. Genename: AIF1
  • 应用备注

    Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 16 kDa band observed in Rat Brain lysates (calculatedMW of 16.6 kDa according to Human NP_001614.3 and of 16.6 kDa according to RatNP_058892.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

    别名

    AIF1

    背景

    Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

    基因ID

    199

    NCBI登录号

    NP_116573

    UniProt

    P55008

    途径

    Smooth Muscle Cell Migration
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