PEBP1 抗体 (C-Term)
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Quick Overview for PEBP1 抗体 (C-Term) (ABIN374198)
抗原
See all PEBP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-DYVPKLYEQL SGK
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特异性
- This antibody detects PBP / RKIP at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human, Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_002558.1 Genename: PEBP1
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应用备注
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Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 22 kDa band observed in lysates of Human Kidneyfibroblast HEK293, Human lung carcinoma A549 and Human Hepatoblastoma HepG2 celllines (calculated MW of 21.2 kDa according to NP_002558.1). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))
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别名
- PEBP1 / RKIP
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背景
- PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.Synonyms: HCNPpp, PBP, PEBP, PEBP-1, Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, Raf kinase inhibitor protein
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基因ID
- 5037
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NCBI登录号
- NP_002558
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UniProt
- P30086
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途径
- Feeding Behaviour
抗原
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