MARK4 抗体 (C-Term)
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北京 101111
Quick Overview for MARK4 抗体 (C-Term) (ABIN374191)
抗原
See all MARK4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- IKRKPPPGCS DSPGV
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特异性
- This antibody detects MARK4 at C-term.
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_113605.2. Genename: MARK4
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应用备注
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Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1 μg/mL. Approx 70 kDa band observed in human prostate lysates(calculated MW of 75.0 kDa according to NP_113605.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- MARK4 (MAP/microtubule Affinity-Regulating Kinase 4 (MARK4))
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别名
- MARK4
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背景
- MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.Synonyms: MAP microtubule affinity-regulating kinase 4, MARKL1
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基因ID
- 57787
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NCBI登录号
- NP_113605
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UniProt
- Q96L34
抗原
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