FXYD5 抗体 (C-Term)
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Quick Overview for FXYD5 抗体 (C-Term) (ABIN374185)
抗原
See all FXYD5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- GKCRQLSRLC RNHCR
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特异性
- This antibody detects FXYD5 at C-term.
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_054883.3, NP_659003.1, NP_001158077.1 Genename: FXYD5
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anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (N-Term) antibody
FXYD5 适用: 人, 小鼠, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 22-145) antibodyFXYD5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibodyVerified FXYD5 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 54-103) antibodyFXYD5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 66-115) antibodyFXYD5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 108-157) antibodyFXYD5 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (N-Term) antibodyFXYD5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 70-100), (Middle Region) antibodyFXYD5 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (AA 71-100) antibodyFXYD5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 38 kDa band in Human Spleenlysate and an approx 26 kDa in Human Lung lysate after 1 μg/mL antibody staining. Thebands were successfully blocked by incubation with the immunizing peptide. Please notethat according to the literature this protein is O-glycosylated (PMID: 12672699), which mayexplain the different sizes of bands we observe given the calculated size of 19.5 kDaaccording to NP_054883 and NP_659003. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- FXYD5 (FXYD Domain Containing Ion Transport Regulator 5 (FXYD5))
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别名
- Dysadherin / FXYD5
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背景
- Dysadherin (FXYD5) is a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD. This protein is a negative regulator of calcium-dependent cell-cell adhesion and is implicated in the biogenesis of microvilli.Synonyms: DYSAD, FXYD domain-containing ion transport regulator 5, IWU1
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基因ID
- 53827
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NCBI登录号
- NP_054883
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UniProt
- Q96DB9
抗原
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