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DKK3 抗体 (C-Term)

This anti-DKK3 antibody is a 山羊 多克隆 antibody detecting DKK3 in WB. Suitable for 人.
产品编号 ABIN374182
发货至: 中国

Quick Overview for DKK3 抗体 (C-Term) (ABIN374182)

抗原

See all DKK3 抗体
DKK3

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This DKK3 antibody is un-conjugated

应用范围

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Western Blotting (WB)
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    C-Term

    序列

    C-AAAALLGGEE I

    特异性

    This antibody detects Dickkopf-3 at C-term.

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1 Genename: DKK3
  • 应用备注

    Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx.70 kDa band observed in lysates of cell line HEK293(calculated MW of 38.0 kDa according to NP_037385.2, NP_056965.3 andNP_001018067.1). The observed molecular weight corresponds to earlier findings inliterature (Krupnik et al, Gene.1999 Oct 1,238(2): 301-13. , PMID: 10570958 ).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • 抗原

    DKK3

    别名

    Dickkopf-3 (DKK3)

    背景

    DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC

    基因ID

    27122

    NCBI登录号

    NP_037385

    UniProt

    Q9UBP4
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