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PLK4 抗体 (N-Term)

The 山羊 多克隆 anti-PLK4 antibody (ABIN374175) specifically detects PLK4 in WB. The antibody is reactive with 人, Cow 和 犬 samples.
产品编号 ABIN374175
发货至: 中国
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Quick Overview for PLK4 抗体 (N-Term) (ABIN374175)

抗原

See all PLK4 抗体
PLK4 (Polo-Like Kinase 4 (PLK4))

适用

人, Cow, 犬

宿主

  • 19
  • 3
山羊

克隆类型

  • 20
  • 2
多克隆

标记

  • 14
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PLK4 antibody is un-conjugated

应用范围

  • 8
  • 7
  • 4
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB)
  • 抗原表位

    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    序列

    ATCIGEKIED FKVGN

    特异性

    This antibody detects SAK / STK18 at N-term.

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from N Terminus of the protein sequence according to NP_055079.3 Genename: PLK4
  • 应用备注

    Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 95 kDa band observed in Human Placenta lysates(calculated MW of 105 kDa according to NP_055079.2). An additional faint band of 55 kDawas consistently observed, however this band was not blocked by the immunizing peptideand it is therefore a non-specific signal. We call for caution when used for other assaysthan Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • 抗原

    PLK4 (Polo-Like Kinase 4 (PLK4))

    别名

    PLK4 / SAK / STK18

    物质类

    Phage Protein

    背景

    Synonyms: PLK-4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase PLK4, Serine/threonine-protein kinase Sak

    基因ID

    10733

    NCBI登录号

    NP_055079

    UniProt

    O00444

    途径

    M Phase
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