ELMO1 抗体 (C-Term)
(4 references)Quick Overview for ELMO1 抗体 (C-Term) (ABIN374159)
抗原
See all ELMO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- PKEPSNYDFV YDCN
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特异性
- This antibody is expected to recognise both Human isoforms of this protein (NP_569709.1 and NP_001034548.1 represent the same isoform).
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Bovine, Zebrafish. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C-Terminus of the protein sequence according to NP_055615.8, NP_569709.1, NP_001034548.1 Genename: ELMO1
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anti-Engulfment and Cell Motility 1 (ELMO1) (C-Term) antibody
ELMO1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (C-Term) antibodyVerified ELMO1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) antibodyELMO1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) antibodyELMO1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (AA 372-421) antibodyELMO1 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 犬, 豚鼠, 马, 兔, Bat, 小鸡, Hamster, 猴, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (N-Term) antibodyELMO1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (AA 527-727) antibodyELMO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (AA 520-727) antibodyELMO1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) antibodyELMO1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Engulfment and Cell Motility 1 (ELMO1) (N-Term) antibodyELMO1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: Recommended for use at 1-2 μg/mL. Approx 70 kDa and 50 kDa bands seen inJurkat cell lysate. Note the smaller isoform of this protein has a consensus glycosylationsite, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B. D and Rickwood, D. ,eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin." in: Development (Cambridge, England), Vol. 142, Issue 1, pp. 174-84, (2014) (PubMed).
: "Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 7, pp. 1153-65, (2012) (PubMed).
: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).
: "Lipid rafts mediate internalization of beta1-integrin in migrating intestinal epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G965-76, (2008) (PubMed).
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: "The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin." in: Development (Cambridge, England), Vol. 142, Issue 1, pp. 174-84, (2014) (PubMed).
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- ELMO1 (Engulfment and Cell Motility 1 (ELMO1))
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别名
- ELMO1
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背景
- ELMO1 is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. ELMO1 acts in association with DOCK1 and CRK and was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. It is belived to enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. ELMO1 interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. It is part of a complex with DOCK1 and RAC1 and with DOCK1 and CRK isoform CRK-II. Recent studies show that it interacts with PLEKHG6.Synonyms: CED-12 homolog, ELMO 1, ELMO-1, Engulfment and cell motility protein 1, KIAA0281
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基因ID
- 9844
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NCBI登录号
- NP_055615
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UniProt
- Q92556
抗原
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