Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in human kidney, liver andpancreas lysates (calculated MW of 31.7 kDa according to NP_036269.1). The observedmolecular weight corresponds to earlier findings in literature with different antibodies(Pullamsetti et al, FASEB J.2005 Jul,19(9): 1175-7, PMID: 15827267). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) family. This enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.Synonyms: DDAH, DDAH-1, DDAHI, Dimethylargininase-1, Dimethylarginine dimethylaminohydrolase 1, N(G), N(G)-dimethylarginine dimethylaminohydrolase 1