ADAM12 抗体 (N-Term)
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Quick Overview for ADAM12 抗体 (N-Term) (ABIN372949)
抗原
See all ADAM12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody reacts to A Disintegrin And Metalloproteinase Domain 12 (ADAM12).
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Peptide sequence AARPLPVSPARALC from the N-Terminal region of ADAM-12
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anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 589-738) antibody
ADAM12 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 513-767) antibodyADAM12 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) antibodyADAM12 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 208-304) antibodyADAM12 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 730-909) antibodyADAM12 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (N-Term) antibodyVerified ADAM12 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 201-300) antibodyADAM12 适用: 人, 小鼠 ELISA, WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (N-Term) antibodyADAM12 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ADAM Metallopeptidase Domain 12 (ADAM12) (AA 208-304) antibodyADAM12 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections: 3.75 μg/mL. Western blot: 0.1 - 0.3 μg/mL. ELISA: 1/64000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
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别名
- ADAM12 / MLTN
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背景
- ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.Synonyms: Disintegrin and metalloproteinase domain-containing protein 12, Meltrin alpha
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基因ID
- 8038
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NCBI登录号
- NP_003465
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UniProt
- O43184
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途径
- EGFR Signaling Pathway
抗原
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