HPSE 抗体
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- 抗原 See all HPSE 抗体
- HPSE (Heparanase (HPSE))
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HPSE antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognizes the 65 kDa precursor as well as the 55 kDa and 5 kDa subunits of Human or Mouse Heparanase.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. - 纯化方法
- Protein G Chromatography.
- 免疫原
- Human 50kD-8kD Heparanase Heterodimer.
- 亚型
- IgG
- Top Product
- Discover our top product HPSE Primary Antibody
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anti-Heparanase (HPSE) (AA 353-464) antibody
HPSE 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 40-188) antibodyHPSE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 34-115) antibodyHPSE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 280-412) antibodyHPSE 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 400-543) antibodyHPSE 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Heparanase (HPSE) antibodyHPSE 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 68-117) antibodyHPSE 适用: 人, 小鼠, 大鼠, 马, Cow, 犬, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 36-275) antibodyHPSE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 263-523) antibodyHPSE 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 72-170) antibodyHPSE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
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- 应用备注
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Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This HPSE antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody Rabbit IgG, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 0.001 % Thimerosal as preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- HPSE (Heparanase (HPSE))
- 别名
- Heparanase / HPSE (HPSE 产品)
- 别名
- im:7144134 antibody, hpa antibody, hpa1 antibody, hpr1 antibody, hpse1 antibody, hse1 antibody, HPSE antibody, HPA antibody, HPA1 antibody, HPR1 antibody, HPSE1 antibody, HSE1 antibody, Hpa antibody, Hpr1 antibody, Hep antibody, heparanase antibody, HPSE antibody, hpse antibody, Hpse antibody
- 背景
- Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.Synonyms: Endo-Glucoronidase, HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1
- 基因ID
- 10855
- NCBI登录号
- NP_001092010
- UniProt
- Q9Y251
- 途径
- Glycosaminoglycan Metabolic Process
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