GOT1 抗体 (Internal Region)
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北京 101111
Quick Overview for GOT1 抗体 (Internal Region) (ABIN372773)
抗原
See all GOT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Recognizes Cytoplasmic Aspartate Aminotransferase (GOT1).
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交叉反应 (详细)
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Species reactivity (expected):Rat.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic peptide (C-RSYRYWDAEKR) from an internal region of Human GOT1.
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亚型
- IgG
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应用备注
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ELISA: 1/4000. Western Blot: 0.01-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
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别名
- GOT1
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背景
- Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.Synonyms: Aspartate aminotransferase, Glutamate oxaloacetate transaminase 1, Transaminase A
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分子量
- 46 kDa.
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基因ID
- 2805
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NCBI登录号
- NP_002070
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UniProt
- P17174
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途径
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
抗原
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