Tyrosine Hydroxylase 抗体 (pSer31)
(2 references)Quick Overview for Tyrosine Hydroxylase 抗体 (pSer31) (ABIN372737)
抗原
See all Tyrosine Hydroxylase (TH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer31
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特异性
- Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.
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交叉反应 （详细）
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Species reactivity (expected):Non-Human Primates.
Species reactivity (tested):Mouse and Rat. -
纯化方法
- Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat Tyrosine Hydroxylase (TH).
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亚型
- IgG
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anti-Tyrosine Hydroxylase (TH) (AA 101-165) antibody
TH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (AA 82-310) antibodyTH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (pSer40) antibodyTH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (pSer31) antibodyTH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) antibodyTH 适用: 人 WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (AA 486-514) antibodyTH 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (AA 34-301) antibodyTH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (pSer19) antibodyTH 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (AA 193-222), (Middle Region) antibodyTH 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tyrosine Hydroxylase (TH) (C-Term) antibodyVerified TH 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blotting: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).
: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).
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: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).
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- Tyrosine Hydroxylase (TH)
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别名
- Tyrosine 3-Monooxygenase (TH)
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背景
- Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.Synonyms: TYH, Tyrosine 3-hydroxylase
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基因ID
- 25085
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NCBI登录号
- NP_036872
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UniProt
- P04177
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途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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