Tryptophan Hydroxylase 2 抗体 (pSer19)
Quick Overview for Tryptophan Hydroxylase 2 抗体 (pSer19) (ABIN372731)
抗原
See all Tryptophan Hydroxylase 2 (TPH2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer19
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特异性
- Specific for the ~55k Tryptophan Hydroxylase protein phosphorylated at Ser19.
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交叉反应 （详细）
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Species reactivity (expected):Bovine, Mouse and Zebrafish.
Species reactivity (tested):Rat. -
纯化方法
- Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat Tryptophan Hydroxylase 2 (TPH2).
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亚型
- IgG
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anti-Tryptophan Hydroxylase 2 (TPH2) (Internal Region) antibody
Verified TPH2 适用: 人 ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) (AA 44-225) antibodyTPH2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) antibodyMicroarray verified TPH2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB425277 unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) antibodyTPH2 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) antibodyMicroarray verified TPH2 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB822648 unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) (AA 1-170) antibodyTPH2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) (AA 16-29) antibodyVerified TPH2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) (Internal Region) antibodyVerified TPH2 适用: 小鼠 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) antibodyMicroarray verified TPH2 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB26484 unconjugated
anti-Tryptophan Hydroxylase 2 (TPH2) antibodyMicroarray verified TPH2 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB282025 unconjugated
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应用备注
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Western blotting: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Tryptophan Hydroxylase 2 (TPH2)
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别名
- Tryptophan 5-Hydroxylase 2 (TPH2)
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背景
- Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2
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基因ID
- 317675
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NCBI登录号
- NP_776211
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UniProt
- Q8CGU9
抗原
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