Tryptophan Hydroxylase 1 抗体
Quick Overview for Tryptophan Hydroxylase 1 抗体 (ABIN372730)
抗原
See all Tryptophan Hydroxylase 1 (TPH1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Specific for the ~55k Tryptophan Hydroxylase protein. The antibody has been directly tested for reactivity in Western blots with Human and Rat tissue. Based upon the relatively high degree of homology of Tryptophan Hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in Rabbit tissues.
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交叉反应 （详细）
- Species reactivity (tested):Human and Rat.
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纯化方法
- Affinity Chromatography.
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免疫原
- Recombinant rabbit Tryptophan Hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
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亚型
- IgG
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anti-Tryptophan Hydroxylase 1 (TPH1) (AA 35-62), (N-Term) antibody
TPH1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB21510 unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 257-405) antibodyTPH1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 345-444) antibodyTPH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (N-Term) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 26-75) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 231-280) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) antibodyTPH1 适用: 人 WB, IHC, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer260) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibodyTPH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1/1,000. Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Tryptophan Hydroxylase 1 (TPH1)
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别名
- Tryptophan 5-Hydroxylase 1 (TPH1)
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背景
- Tryptophan Hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1
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基因ID
- 7166
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NCBI登录号
- NP_004170
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UniProt
- P17752
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途径
- Feeding Behaviour
抗原
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