GRIN2B 抗体 (pTyr1472)
(3 references)Quick Overview for GRIN2B 抗体 (pTyr1472) (ABIN372687)
抗原
See all GRIN2B 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pTyr1472
-
特异性
- This antibody is specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either lambda-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
-
交叉反应 （详细）
-
Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, non-Human Primates and Zebrafish.
Species reactivity (tested):Rat. -
纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
-
免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.
-
亚型
- IgG
-
-
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 27-163) antibody
GRIN2B 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6E9A8 unconjugated
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (HRP)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 HRP
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (FITC)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 FITC
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (Biotin)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 Biotin
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (PE)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 PE
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (APC)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 APC
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (PerCP)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 PerCP
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (Atto 488)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 Atto 488
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (Atto 594)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 Atto 594
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (AA 20-271) antibody (Atto 390)GRIN2B 适用: 大鼠 WB, IHC, IF, IP, ICC, AA 宿主: 小鼠 Monoclonal S59 Atto 390
-
-
应用备注
-
Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
-
-
-
: "Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B." in: The Journal of experimental medicine, Vol. 213, Issue 13, pp. 2949-2966, (2017) (PubMed).
: "Calpain and STriatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington's disease mouse model." in: Human molecular genetics, Vol. 21, Issue 17, pp. 3739-52, (2012) (PubMed).
: "Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20942-56, (2012) (PubMed).
-
: "Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B." in: The Journal of experimental medicine, Vol. 213, Issue 13, pp. 2949-2966, (2017) (PubMed).
-
- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
-
别名
- NMDAR2B
-
背景
- The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a, Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001, Roche et al., 2001).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B
-
基因ID
- 2904
-
NCBI登录号
- NP_000825
-
UniProt
- Q13224
-
途径
- Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
抗原
-
