STXBP1 抗体 (pSer515)
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Quick Overview for STXBP1 抗体 (pSer515) (ABIN372671)
抗原
See all STXBP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer515
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特异性
- This antibody recognizes ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
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纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser515 of Munc-18.
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亚型
- IgG
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应用备注
- Western blot: 1/1000. Immunohistochemistry: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20°C.
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有效期
- 12 months
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- STXBP1 (Syntaxin Binding Protein 1 (STXBP1))
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别名
- STXBP1 / UNC18A
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背景
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Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Alternate names: N-Sec1, Syntaxin-binding protein 1, Unc-18 homolog, Unc-18-1, Unc-18A -
基因ID
- 25558
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NCBI登录号
- NP_037170
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UniProt
- P61765
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途径
- Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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