MARCKS 抗体 (pSer152, pSer156)
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北京 101111
Quick Overview for MARCKS 抗体 (pSer152, pSer156) (ABIN372663)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer152, pSer156
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交叉反应 (详细)
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Species reactivity (expected):Bovine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat. -
纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser152,156 of MARCKS.
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亚型
- IgG
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应用备注
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Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
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基因ID
- 10116
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UniProt
- P30009
抗原
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