Western blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at-20 °C.
抗原
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
别名
MARCKS
背景
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL