Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
Specific for the ~95k Dynamin protein phosphorylated at Ser774. This antiboy labels the purified protein phosphorylated in vitro by CDK5 but not by PKC. Does not cross react with other purified substrates of CDK5 (e.g. Amphiphysin and Synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
交叉反应 (详细)
Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates. Species reactivity (tested):Rat.
纯化方法
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
免疫原
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat Dynamin.
Western Blot: 1/1000. Immunohistochemistry (Fixed Cultured Neurons): 1/1000. (See Ref.3)Immunofluorescence (Fixed Cultured Neurons): 1/1000. (See Ref.3) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at-20 °C.
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates Dynamin at Ser774 and Ser778 that are the phosphorylation sites on Dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on Dynamin is thought to play a key role in synaptic vesicle trafficking.Synonyms: DNM, DNM1