CDK1 抗体 (pTyr15)
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Quick Overview for CDK1 抗体 (pTyr15) (ABIN372596)
抗原
See all CDK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr15
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特异性
- Specific for the ~38k CDC2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat and Zebrafish.
Species reactivity (tested):Human and Xenopus. -
纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr15 of rat CDC2.
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亚型
- IgG
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应用备注
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Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- CDK1
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背景
- Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle (Maller, 1991). The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2 (Nakamizo et al., 2002). cdc2 expression in brain has been linked to both neurogenesis and apoptosis (Konishi and Bonni, 2003, Dranovsky et al., 2001, Okano et al., 1996).Synonyms: CDC2, CDC28A, CDK1, CDKN1, Cell division control protein 2 homolog, Cell division protein kinase 1, Cyclin-dependent kinase 1, P34CDC2, p34 protein kinase
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基因ID
- 983
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NCBI登录号
- NP_001777
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UniProt
- P06493
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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