AQP2 抗体 (pSer261)
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北京 101111
Quick Overview for AQP2 抗体 (pSer261) (ABIN372590)
抗原
See all AQP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer261
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特异性
- Specific for ~29k AQP2 protein phosphorylated at Ser261. This antibody Also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
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交叉反应 （详细）
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Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat. -
纯化方法
- Affinity Chromatography.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of Rat Aquaporin 2.
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亚型
- IgG
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anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody
AQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-271) antibodyAQP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 147-271) antibodyAQP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 149-271) antibodyAQP2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8B5 unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 241-271), (C-Term) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 245-260) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibodyAQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 101-200) antibodyAQP2 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (pSer269) antibodyAQP2 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Aquaporin 2 (Collecting Duct) (AQP2) (C-Term) antibody (HRP)AQP2 适用: 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
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应用备注
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Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).
: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).
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: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).
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- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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别名
- Aquaporin-2
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背景
- Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct
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基因ID
- 25386
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NCBI登录号
- NP_037041
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UniProt
- P34080
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途径
- Response to Water Deprivation
抗原
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