YWHAB 抗体 (pSer58)
Quick Overview for YWHAB 抗体 (pSer58) (ABIN372585)
抗原
See all YWHAB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer58
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特异性
- Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
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交叉反应 （详细）
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Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Sheep, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Human and Rat. -
纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat 14-3-3 protein.
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亚型
- IgG
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anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibody
YWHAB 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 5B5G10 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (Center) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-141) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 34-221) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (pSer58) antibodyYWHAB 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 56-85), (N-Term) antibodyYWHAB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4062 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 217-246), (C-Term) antibodyYWHAB 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4064 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 51-150) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- YWHAB (14-3-3 alpha + beta (YWHAB))
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别名
- 14-3-3 Protein beta/alpha
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背景
- 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003).Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
抗原
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