YWHAB 抗体 (C-Term)
Quick Overview for YWHAB 抗体 (C-Term) (ABIN372584)
抗原
See all YWHAB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.
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交叉反应 （详细）
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Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat. -
纯化方法
- Affinity Chromatography.
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免疫原
- Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
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亚型
- IgG
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anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibody
YWHAB 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 5B5G10 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (Center) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-246) antibodyYWHAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 1-141) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 34-221) antibodyYWHAB 适用: 人, 斑马鱼 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (pSer58) antibodyYWHAB 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 56-85), (N-Term) antibodyYWHAB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4062 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 217-246), (C-Term) antibodyYWHAB 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4064 unconjugated
anti-14-3-3 alpha + beta (YWHAB) (AA 51-150) antibodyYWHAB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- YWHAB (14-3-3 alpha + beta (YWHAB))
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别名
- 14-3-3 Protein beta/alpha
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背景
- 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004, Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.Synonyms: KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB
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基因ID
- 56011
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NCBI登录号
- NP_062250
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UniProt
- P35213
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
抗原
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