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- 抗原 See all Bu-1b products
- Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))
- 适用
- 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Bu-1b antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 产品特性
- Synonyms: B6.2, Bu1b
- 纯化方法
- Purified
- 免疫原
- Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.
- 克隆位点
- 5-11G2
- 亚型
- IgG1
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anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (PE)
Bu-1b 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 5-11G2 PE
anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibodyBu-1b 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 5-11G2 unconjugated
anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (Biotin)Bu-1b 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 5-11G2 Biotin
anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (FITC)Bu-1b 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 5-11G2 FITC
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- 应用备注
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Flow Cytometry: < / = 1 μg/10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))
- 别名
- Bu-1b (Bu-1b 产品)
- 背景
- Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90 % of bursal cells, 2 to 8 % of thymocytes, 15 to 27 % of spleen cells, and 2 to 18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.Synonyms: B6.2, chicken B-cell marker
- UniProt
- Q90747
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