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- 抗原 See all BU-1A / B6.1 products
- BU-1A / B6.1
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适用
- 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BU-1A / B6.1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence.
- 产品特性
- Synonyms: B-Cell Marker, BU-1 Allele A
- 纯化方法
- Purified
- 免疫原
- Bursal cells from one-day-old H. B15 (Bu-1a/b) strain chickens.
- 克隆位点
- 21-1A4
- 亚型
- IgG1
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anti-BU-1A / B6.1 antibody (PE)
适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 21-1A4 PE
anti-BU-1A / B6.1 antibody适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 21-1A4 unconjugated
anti-BU-1A / B6.1 antibody (Biotin)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 21-1A4 Biotin
anti-BU-1A / B6.1 antibody (FITC)适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 21-1A4 FITC
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- 应用备注
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Flow Cytometry: < / = 1 μg/10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- BU-1A / B6.1
- Abstract
- BU-1A / B6.1 产品
- 背景
- Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.Synonyms: B-Cell Marker, BU-1 Allele A
- 基因ID
- 396098
- UniProt
- Q90746
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