MHC Class I H-2Db 抗体
The 小鼠 单克隆 anti-MHC Class I H-2Db antibody is suitable to detect MHC Class I H-2Db in samples from 小鼠. It has been validated for FACS.
产品编号 ABIN371165
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class I H-2Db 抗体 (ABIN371165)
抗原
MHC Class I H-2Db
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS)
克隆位点
28-14-8
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特异性
- This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.
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产品特性
- Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain
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内毒素水平
- Low Endotoxin
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免疫原
- C3H. SW mouse splenocytes. (Ref. 1,2)
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亚型
- IgG2a
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应用备注
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Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Tissue Sections. Complement-mediated cytotoxicity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS without preservatives.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- MHC Class I H-2Db
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别名
- MHC Class I H-2 Db
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背景
- The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1
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基因ID
- 14964
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UniProt
- P01899
抗原
-
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