MBP 抗体
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北京 101111
Quick Overview for MBP 抗体 (ABIN364503)
抗原
See all MBP 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
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纯化方法
- Ammonium sulfate precipitation
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免疫原
- Human myelin basic protein from brain.
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亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (1:100), WB (1:1500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1500 in TTBS, overnight at 4uC. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.05M Tris HCL, pH 7.6, 15 mM sodium azide before the addition of glycerol to 40 %
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MBP (Myelin Basic Protein (MBP))
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别名
- Myelin Basic Protein / MBP
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背景
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Name/Gene ID: MBP
Synonyms: MBP, Myelin A1 protein, Myelin basic protein -
基因ID
- 4155
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UniProt
- P02686
抗原
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