Laminin 抗体
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北京 101111
Quick Overview for Laminin 抗体 (ABIN364494)
抗原
See all Laminin (LN) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Typically less than 1 % cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible
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纯化方法
- Immunoaffinity purified
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免疫原
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Mixture of synthetic peptides corresponding to regions of adherence on laminin.
Type of Immunogen: Synthetic peptide
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应用备注
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Approved: ELISA (1:4000 - 1:8000), IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
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- Laminin (LN)
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别名
- Laminin
抗原
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