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Laminin 抗体

The 兔 多克隆 anti-Laminin antibody (ABIN364494) specifically detects Laminin in IHC, ELISA 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN364494
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Laminin 抗体 (ABIN364494)

抗原

See all Laminin (LN) 抗体
Laminin (LN)

适用

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宿主

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克隆类型

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多克隆

标记

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This Laminin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    Typically less than 1 % cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible

    纯化方法

    Immunoaffinity purified

    免疫原

    Mixture of synthetic peptides corresponding to regions of adherence on laminin.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (1:4000 - 1:8000), IHC, IHC-P (5 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
  • 抗原

    Laminin (LN)

    别名

    Laminin
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