HSPB6 抗体
Quick Overview for HSPB6 抗体 (ABIN364293)
抗原
See all HSPB6 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes HSP20, Mr ~20kD. Species cross-reactivity: Human, mouse, rabbit, rat and bovine.
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纯化方法
- Immunoaffinity purified
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免疫原
- Native HSP20 purified from human skeletal muscle.
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亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (2.5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot Analysis: 1 μg/mL detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol. Immunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M PBS, pH 7.0, 0.1 % sodium azide, 30 mg/mL BSA, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))
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别名
- HSPB6 / HSP20
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背景
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Name/Gene ID: HSPB6
Synonyms: HSPB6, Heat shock protein beta-6, Hsp20 -
基因ID
- 126393
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UniProt
- O14558
抗原
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