ITGB1 抗体
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- 抗原 See all ITGB1 抗体
- ITGB1 (Integrin beta 1 (ITGB1))
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适用
- 人, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))
- 品牌
- IHC-plus™
- 特异性
- Specific for active conformation of human b1 integrins. Does not augment the affinity of beta 1 for ligands. Species cross-reactivity: rat.
- 纯化方法
- Purified
- 免疫原
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Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
Type of Immunogen: Cells - 克隆位点
- HUTS-4
- 亚型
- IgG2b
- Top Product
- Discover our top product ITGB1 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, IHC, IHC-P (10 μg/mL), IHC-Pfa, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. FACS: 2.5 μg/5x10^5 cells. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.1. No preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- ITGB1 (Integrin beta 1 (ITGB1))
- 别名
- ITGB1 / Integrin Beta 1 / CD29 (ITGB1 产品)
- 背景
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Name/Gene ID: ITGB1
Family: Integrin
Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta - 基因ID
- 3688
- UniProt
- P05556
- 途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
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