CDK1 抗体 (AA 13-17)
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Quick Overview for CDK1 抗体 (AA 13-17) (ABIN362907)
抗原
See all CDK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-17
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特异性
- The antibody detects endogenous level of total CDC2 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity CDC2 (Ab-15) antibody detects endogenous levels of total CDC2 protein.
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免疫原
- Peptide sequence around AA 13-17 (G-T-Y-G-V) derived from Human CDC2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents." in: The Biochemical journal, Vol. 446, Issue 3, pp. 373-81, (2012) (PubMed).
: "SUMOylation-regulated protein phosphorylation, evidence from quantitative phosphoproteomics analyses." in: The Journal of biological chemistry, Vol. 286, Issue 31, pp. 27342-9, (2011) (PubMed).
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- CDC2
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背景
- Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
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分子量
- 34 kDa
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NCBI登录号
- NP_001163
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UniProt
- P06493
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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