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EIF4E 抗体 (AA 207-211)

The 兔 多克隆 anti-EIF4E antibody is suitable to detect EIF4E in samples from 人, 大鼠 和 小鼠. It has been validated for WB, IHC 和 IF.
产品编号 ABIN362892
发货至: 中国
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Quick Overview for EIF4E 抗体 (AA 207-211) (ABIN362892)

抗原

See all EIF4E 抗体
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

适用

  • 145
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  • 20
  • 5
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人, 大鼠, 小鼠

宿主

  • 130
  • 15
  • 2

克隆类型

  • 99
  • 48
多克隆

标记

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This EIF4E antibody is un-conjugated

应用范围

  • 126
  • 68
  • 51
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  • 27
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  • 16
  • 7
  • 6
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

    • 53
    • 15
    • 8
    • 7
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    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
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    • 1
    • 1
    • 1
    AA 207-211

    特异性

    The antibody detects endogenous level of total elF4E protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

    免疫原

    Peptide sequence around AA 207-211 (S-G-S-T-T) derived from Human eIF4E. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    别名

    eIF4E

    背景

    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

    分子量

    25 kDa

    NCBI登录号

    NP_001124

    UniProt

    P06730

    途径

    BCR Signaling
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