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VASP 抗体 (AA 155-159)

The 兔 多克隆 anti-VASP antibody is suitable to detect VASP in samples from 人 和 小鼠. It has been validated for WB 和 IF.
产品编号 ABIN362856
发货至: 中国
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Quick Overview for VASP 抗体 (AA 155-159) (ABIN362856)

抗原

See all VASP 抗体
VASP (Vasodilator-Stimulated phosphoprotein (VASP))

适用

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人, 小鼠

宿主

  • 120
  • 17
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克隆类型

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  • 36
多克隆

标记

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This VASP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    AA 155-159

    特异性

    The antibody detects endogenous level of total VASP protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

    免疫原

    Peptide sequence around AA 155-159 (R-V-S-N-A) derived from Human VASP. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    VASP (Vasodilator-Stimulated phosphoprotein (VASP))

    别名

    VASP

    背景

    Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

    分子量

    50 kDa

    NCBI登录号

    NP_003361

    UniProt

    P50552

    途径

    TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
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