The antibody detects endogenous level of total HDAC4 protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
免疫原
Peptide sequence around AA 630-634 (A-Q-S-S-P) derived from Human HDAC4. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
抗原
HDAC4
(Histone Deacetylase 4 (HDAC4))
别名
HDAC4
背景
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.