HDAC4/HDAC5/HDAC9 抗体 (pSer246)
Quick Overview for HDAC4/HDAC5/HDAC9 抗体 (pSer246) (ABIN362452)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer246
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特异性
- The antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer246, 259, 220 (T-A-S (p) -EP) derived from Human HDAC4, HDAC5, HDAC9. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-HDAC4,HDAC5,HDAC9 antibody
适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HDAC4,HDAC5,HDAC9 (pSer220), (pSer246), (pSer259) antibody适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HDAC4,HDAC5,HDAC9 (Ser220), (Ser246), (Ser259) antibody适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-HDAC4,HDAC5,HDAC9 (pSer220), (pSer246), (pSer259) antibody适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- HDAC4/HDAC5/HDAC9 (HDAC4,HDAC5,HDAC9)
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别名
- HDAC4/HDAC5/HDAC9
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背景
- Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs, classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
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分子量
- 124 kDa
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NCBI登录号
- NP_055522, NP_001015
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UniProt
- Q9UKV0
抗原
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