EIF4G1 抗体 (pSer1232)
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Quick Overview for EIF4G1 抗体 (pSer1232) (ABIN362450)
抗原
See all EIF4G1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer1232
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特异性
- The antibody detects endogenous level of eIF4G only when phosphorylated at serine 1232.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer1232 (P-V-S (p) -P-L) derived from Human eIF4G. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1500-1599) antibody
EIF4G1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1500-1599) antibodyEIF4G1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) antibodyEIF4G1 适用: 人 IHC, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (pSer1232) antibodyEIF4G1 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1-645) antibodyEIF4G1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 497-681) antibodyEIF4G1 适用: 人, 小鼠, 大鼠 IHC, WB, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (Ser1232) antibodyEIF4G1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 1500-1599) antibodyEIF4G1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 400-500) antibodyEIF4G1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1) (AA 539-588) antibodyEIF4G1 适用: 人, 小鼠, 大鼠, Cow, 猴, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))
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别名
- eIF4G
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背景
- EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3, in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
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分子量
- 220 kDa
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NCBI登录号
- NP_004944
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UniProt
- Q04637
抗原
-
