The antibody detects endogenous level of cofilin1 only when phosphorylated at tyrosine 139.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pTyr139 (N-C-Y (p) -E-E) derived from Human cofilin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
抗原
Cofilin (CFL1)
(Cofilin 1 (CFL1))
别名
cofilin1
背景
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.