JNK1/2 抗体 (pThr183, pTyr185)
This 兔 多克隆 antibody specifically detects JNK1/2 in WB 和 IF. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN362437
发货至:
中国
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Quick Overview for JNK1/2 抗体 (pThr183, pTyr185) (ABIN362437)
抗原
JNK1/2
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This JNK1/2 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunofluorescence (IF)
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抗原表位
- pThr183, pTyr185
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特异性
- The antibody detects endogenous level of JNK1/JNK2/JNK3 only when phosphorylated at Thr183/Tyr185.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pThr183, pTyr185 (M-M-T (p) -P-Y (p) - V - V ) derived from Human JNK1, JNK2, JNK3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-JNK1/2 (AA 311-424) antibody
适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- JNK1/2
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别名
- JNK1/JNK2
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背景
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Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity -
分子量
- 54 kDa
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NCBI登录号
- NP_002744, NP_001128
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UniProt
- P53779
抗原
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