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SMAD1 抗体 (pSer465)

This 兔 多克隆 antibody specifically detects SMAD1 in WB. It exhibits reactivity toward 人, 小鼠 和 大鼠.It has been mentioned in 1 publication
产品编号 ABIN362414
发货至: 中国
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Quick Overview for SMAD1 抗体 (pSer465) (ABIN362414)

抗原

See all SMAD1 抗体
SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This SMAD1 antibody is un-conjugated

应用范围

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Western Blotting (WB)
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    pSer465

    特异性

    The antibody detects endogenous level of Smad1 only when phosphorylated at serine 465.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pSer465 (I-S-S-V-S (p) ) derived from Human Smad1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Yamaguchi, Zhu, Yu, Sasaki, Umetsu, Kidachi, Ryoyama et al.: "Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their ..." in: Cell biology international, Vol. 31, Issue 6, pp. 638-44, (2007) (PubMed).

  • 抗原

    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

    别名

    Smad1

    背景

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).

    分子量

    55 kDa

    NCBI登录号

    NP_001003

    UniProt

    Q15797

    途径

    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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