MARCKS 抗体 (pSer162)
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Quick Overview for MARCKS 抗体 (pSer162) (ABIN362334)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer162
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特异性
- The antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer162 (K-K-S (p) -F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
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分子量
- 80 kDa
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NCBI登录号
- NP_002347
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UniProt
- P29966
抗原
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