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EIF4E 抗体 (pSer209)
EIF4E
适用: 人, 大鼠, 小鼠
WB, IHC, IF
宿主: 兔
Polyclonal
unconjugated
EIF4E抗体详情
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抗原
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EIF4E
(Eukaryotic Translation Initiation Factor 4E (EIF4E))
抗原表位
All epitopes for EIF4E 抗体
pSer209
适用
All reactivities for EIF4E 抗体
人, 大鼠, 小鼠
宿主
All hosts for EIF4E 抗体
兔
克隆类型
All clonalities for EIF4E 抗体
多克隆
标记
All conjugates for EIF4E 抗体
This EIF4E antibody is un-conjugated
应用范围
All applications for EIF4E 抗体
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer209 (S-G-S (p) -T-T) derived from Human eIF4E. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
亚型
IgG
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使用细节
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应用备注
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
EIF4E目标详情
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抗原
EIF4E
(Eukaryotic Translation Initiation Factor 4E (EIF4E))
别名
eIF4E (EIF4E 产品 )
别名
AUTS19 antibody, CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, eif4e-1 antibody, zgc:86680 antibody, EG668879 antibody, Eif4e-ps antibody, If4e antibody, eIF-4E antibody, 0260/09 antibody, 0587/11 antibody, 0589/11 antibody, 0919/12 antibody, 1004/13 antibody, 2 antibody, 7238 antibody, CG4035 antibody, CT13384 antibody, CT39424 antibody, CT39426 antibody, D-eIF4E antibody, Dmel\\CG4035 antibody, Eif4E antibody, Eif4e antibody, IF4E antibody, dEif4e antibody, deIF-4E antibody, deIF4E antibody, eIF 4E antibody, eIF-4E1 antibody, eIF-4E2 antibody, eIF-4EII antibody, eIF-4e antibody, eIF4E antibody, eIF4E-1 antibody, eIF4E-1/2 antibody, eIF4E-2 antibody, eIF4E1 antibody, eIF4EI antibody, eIF4e antibody, eif-4E antibody, eif-4e antibody, eif4e antibody, l(3)07238 antibody, l(3)67Af antibody, l(3)S025007 antibody, l(3)S026009 antibody, l(3)S058711 antibody, l(3)S091912 antibody, cbp antibody, eif4f antibody, eif4e1 antibody, eif4el1 antibody, EIF-4E antibody, zgc:101581 antibody, eukaryotic translation initiation factor 4E antibody, eukaryotic translation initiation factor 4ea antibody, Eukaryotic initiation factor 4E antibody, eucaryotic initiation factor 6 antibody, eukaryotic translation initiation factor 4E L homeolog antibody, eukaryotic translation initiation factor 4E family member 1B S homeolog antibody, translation initiation factor eIF4E antibody, eukaryotic translation initiation factor 4eb antibody, EIF4E antibody, eif4ea antibody, Eif4e antibody, eIF-4E antibody, eif6 antibody, eif4e antibody, eif4e.L antibody, eif4e1b.S antibody, LOC100533326 antibody, eif4eb antibody
背景
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
分子量
25 kDa
NCBI登录号
NP_001124
UniProt
P06730
途径
BCR Signaling
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