Tyrosine Hydroxylase 抗体 (pSer40)
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Quick Overview for Tyrosine Hydroxylase 抗体 (pSer40) (ABIN362245)
抗原
See all Tyrosine Hydroxylase (TH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer40
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特异性
- The antibody detects endogenous level of Tyrosine Hydroxylase only when phosphorylated at serine 40.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer40 (R-Q-S (p) -L-I) derived from Human pTyrosine Hydroxylase (TH) . Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
- Immunofluorescence: 1:100-1:200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Tyrosine Hydroxylase (TH)
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别名
- Tyrosine Hydroxylase
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背景
- The protein encoded by Tyrosine Hydroxylase is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
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分子量
- 55-60 kDa
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NCBI登录号
- NP_000351
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UniProt
- P07101
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途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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