The antibody detects endogenous level of HSF1 only when phosphorylated at serine 307.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pSer307 (P-Q-S (p) -P-R) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
HSF1
适用: 人
WB, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
抗原
HSF1
(Heat Shock Factor Protein 1 (HSF1))
别名
HSF1
背景
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.