Histone H3.1 抗体 (pSer10)
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Quick Overview for Histone H3.1 抗体 (pSer10) (ABIN362204)
抗原
See all Histone H3.1 (HIST1H3B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer10
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特异性
- The antibody detects endogenous level of Histone H3.1 only when phosphorylated at serine 10.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer10 (R-K-S (p) -T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-Histone H3.1 (HIST1H3B) antibody
HIST1H3B 适用: 人 WB, IF, ELISA, IHC, FACS 宿主: 兔 Monoclonal 27F2 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (pSer10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (H3K4me2) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC, FACS 宿主: 兔 Monoclonal 6F6 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (meArg17) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3E10 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3S10ph) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2A5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3R2me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 4G5 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (meLys36) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 1F4 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K14ac) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 3H3 unconjugated Recombinant Antibody
anti-Histone H3.1 (HIST1H3B) (H3K18me1) antibodyHIST1H3B 适用: 人 WB, IF, ELISA, ICC 宿主: 兔 Monoclonal 2B5 unconjugated Recombinant Antibody
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." in: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).
: "Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR?/NF-?B pathway." in: Respiratory research, Vol. 13, pp. 110, (2013) (PubMed).
: "The protective effect of alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose." in: Biochemical pharmacology, Vol. 84, Issue 7, pp. 961-73, (2012) (PubMed).
: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." in: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).
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- Histone H3.1 (HIST1H3B)
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别名
- Histone H3.1
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背景
- Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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分子量
- 17 kDa
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NCBI登录号
- NP_003521
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UniProt
- P68431
抗原
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