The antibody detects endogenous level of NMDAR2B only when phosphorylated at Tyr1474.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed bychromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pTyr1474 (H-V-Y (p) -E-K) derived from Human NMDAR2B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.